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. 2015 Dec 3;10(12):e0144100. doi: 10.1371/journal.pone.0144100

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© 2015 Gage et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 6 — (A) RT-qPCR of day 17 and day 26 whole population samples examining the expression of transcription factors believed to be involved in pancreatic endocrine fate specification. Wild type (WT; black bars) and ARX knockout clone 1 (ARX ko; red bars) cell samples relative to adult human islets expression levels. † indicates p < 0.05 within the genotype over time, * indicates p < 0.05 wild type vs ARX ko at given culture day, N = 4 per group. (A-E) Immunostaining of 26-day differentiated wild type and ARX ko cells, 13 week human fetal pancreatic tissue and adult human pancreatic tissue. Inset is a ~3x enlarged portion from the region indicated by the star. Scale bar is 100 μm for all panels.