Table 1. IC50 values of the PNME and standard compounds for different antioxidant, free radical and metal chelation assays.
Name of Assay | PNME | Standards | Values of Standard compounds |
---|---|---|---|
DPPH | 30.92 ± 0.40 | Ascorbic acid | 5.29 ± 0.28 |
Superoxide anion (O2•−) scavenging | 225.73 ± 3.09 | Quercetin | 42.06 ± 1.35 |
Hydroxyl radical (OH•) scavenging | 1162.99 ± 23.05 | Mannitol | 571.45 ± 20.12 |
Hypochlorous acid (HOCl) scavenging | 309.68 ± 18.15 | Ascorbic acid | 235.96 ± 5.75 |
Singlet oxygen (1O2) scavenging | 743.44 ± 13.87 | Lipoic acid | 46.15 ± 1.16 |
Nitric oxide radical (NO) scavenging | 321.25 ± 4.15 | Curcumin | 90.82 ± 4.75 |
Peroxynitrite (ONOO-) scavenging | 2.25 ± 0.42 | Gallic acid | 0.876 ± 0.57 |
Iron chelating activity | 25.64 ± 0.60 | EDTA | 1.27 ± 0.05 |
Lipid peroxidation | 263.75 ± 12.11 | Trolox | 6.76 ± 0.17 |
All the values are expressed in μg/ml excluding in Peroxynitrite scavenging assay where values express in mg/ml. Data expressed as mean ± S.D (n = 6). EDTA represents Ethylenediamine tetraacetic acid.