Fig 4. Proteome-wide analysis of protein sumoylation sites using GG-remnant method and the chemical labeling and Ulp1-cleavage method.

(A) Sumoylated proteins were purified using Ni-NTA and anti-FLAG affinity resins, eluted by denaturing method and then divided for various treatments as indicated. (B) Venn diagram shows the number of sumoylation sites identified by the GG-remnant method, acetylation without Ulp1 cleavage, and acetylation with Ulp1-cleavage method. (C) Sumoylation sites identified with the position of lysine for each protein shown in parenthesis. Previously known sumoylation sites are underlined.