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. 2015 Dec 3;10(12):e0143810. doi: 10.1371/journal.pone.0143810

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© 2015 Albuquerque et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 6 — (A) Effect of uba2-K229R mutations on the ability of Aos1-Uba2 in catalyzing sumoylation reaction. (B) Effect of uba2-K229R and uba2-3KR on Uba2 sumoylation in vivo. Whole cell lysates from the indicated yeast strains were subjected to Western blot analysis. (C) Effect of uba2-K229R and uba2-3KR mutations on cell growth. The mec1Δ mutant is used as a positive control for sensitivity to hydroxyurea (HU), camptothecin (CPT) and UV.