Skip to main content
. Author manuscript; available in PMC: 2015 Dec 3.
Published in final edited form as: Nat Immunol. 2015 Sep 7;16(10):1025–1033. doi: 10.1038/ni.3267

Figure 5.

Figure 5

The interferon response induced by HIV-1 infection correlates with the presence of (−)-strand DNA but not with the presence of (+)-strand DNA. (a) Strand-specific quantitative detection of the HIV-1 (+) strand and (−) strand (key) in cytosol-enriched nucleic acid preparations of wild-type (WT) or cGAS-deficient (cGAS-KO) THP-1 cells 4 h after infection with HIV-1 particles containing wild-type (HIV: WT) or mutant (HIV: N265D) reverse transcriptase or without infection (HIV: −); results are presented as ssDNA copies per mitochondrial genome (mit gen). (b,c) IFIT2 mRNA in THP-1 cells 4 h after treatment as in a (b) or after transfection of genomic DNA or 3P-dsRNA or treatment with medium alone (Med) (c); results are presented as copies of IFIT2 mRNA per copy of control GAPDH mRNA (c), or that value relative to the results of wild-type THP-1 cells infected with wild-type particles (b). (d) Strand-specific quantitative detection of the HIV-1 (+) and (−) strand (key) in cytosol-enriched nucleic acid preparations of monocyte-derived macrophages 4 h after no infection (Med) or infection with HIV-1 particles as in a (presented as in a). (e) Ratio of (+) strand to (−) strand in d. (f,g) IFNB1 mRNA (f) and IFIT2 mRNA (g) in monocyte-derived macrophages 4 h after stimulation with HIV-1 particles as in a or transfection of genomic DNA or 3P-dsRNA; results are presented as copies of IFNB1 mRNA (f) or IFIT2 mRNA (g) per copy of control GAPDH mRNA. *P ≤ 0.01 (ratio-paired t-test). Data are from three (a,b) i or two (c) independent experiments (mean and s.e.m.) or are pooled from two experiments with two biological replicates in each (d–g; mean and s.e.m. of n = 4 donors).