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. Author manuscript; available in PMC: 2015 Dec 3.
Published in final edited form as: Dalton Trans. 2015 Oct 7;44(37):16494–16505. doi: 10.1039/c5dt02689j

Fig. 8.

Fig. 8

Integrated intensity of a representative ITC titration of 1.73 mM GSH into 168 μM Cu+ in 25 mM HEPES, 100 mM NaCl and 25 mM MeCN at pH 7.5 on a TA Instruments Nano ITC. The solid black line indicates the best fit using a one-site binding model (n = 0.98 ± 0.02, K = 2.9 ± 0.1 × 105, ΔH = −108 ± 6 kJ mol−1, RSS/DoF = 158.3) and the red line represents the best fit using a sequential binding model (K1 = 6 ± 5 × 105, ΔH1 = −101 ± 3 kJ mol−1, K2 = 1 ± 8 × 103, ΔH2 = −10 ± 10 kJ mol−1, RSS/DoF = 23.5). Open boxes indicate the heat of GSH dilution.