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. 2015 Sep 17;119(11):1303–1312. doi: 10.1152/japplphysiol.00048.2015

Table 2.

Primer sequences for RT-PCR

Gene Forward Sequence (5′-3′) Reverse Sequence (5′-3′)
PGC-1α CACTTACAAGCCAAACCAACAACT CAATAGTCTTGTTCTCAAATGGGGA
PPARD TGACCTGGCCCTATTCATTG GGAAGAGGTACTGGGCATCA
HIF1A TCTGGGTTGAAACTCAAGCA TCAACCGGTTTAAGGACACA
CS CCTGCCTAATGACCCCATGTT CATAATACTGGAGCAGCACCCC
COX IV CGAGCAATTTCCACCTCTGT GGTCACGCCGATCCATATA
TFAM GGAAGGTCTGGAGCAGAGC TGGACAACTTGCCAAGACAG
PHD2 CGCAACCCTCATGAAGTACA TTACCGACCGAATCTGAAGG
GAPDH CCTCCTGCACCACCAACTGCTT GAGGGGCCATCCACAGTCTTCT

PGC-1α, peroxisome proliferator activated receptor γ co-activator-1α; PPARD, peroxisome proliferator activated receptor delta; HIF1A, hypoxia-inducible factor 1-alpha; CS, citrate synthase; COX IV, cytochrome oxidase subunit IV; TFAM, mitochondrial transcription factor A; PHD2, prolyl hydroxylase 2; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.