Fig. 3.

Cytokine dysregulation of KLF10^−/− macrophages. A: cytokine release by CD11b⁺ colonic mononuclear cells isolated from WT or KLF10^−/− mice. Cells were incubated in medium with or without LPS (100 ng/ml) for 24 h and supernatants were collected and analyzed for the expression of IL-10, TNF-α, and IL-12p70. The data represent means ± SD of 3 independent experiments from 3 different WT or KLF10^−/− mice. NT, not treated; NS, not significant. B: cytokine release by WT or KLF10^−/− BMDM. BMDM were stimulated with LPS plus IFN-γ for 24 h and supernatants were analyzed for the production of TNF-α and IL-6. The data represent means ± SD of 3 independent experiments from 3 different WT or KLF10^−/− mice.