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. 2015 Oct 15;309(11):G900–G909. doi: 10.1152/ajpgi.00309.2015

Fig. 5.

Fig. 5.

KLF10 binds to and transactivates the promoter activity of TGFβRII in macrophages. A: chromatin immunoprecipitation (ChiP) assay demonstrating binding of KLF10 to the TGFβRII core promoter locus in BMDM. Semiquantitative PCR analysis of the expression of TGFβRII postimmunoprecipitation for His-tagged KLF10 in BMDM transfected with KLF10-His expression vector demonstrate significant binding of KLF10 to the core promoter. Results presented are controlled to TGF-βRII expression of preimmunoprecipitated sample (input). Left: representative DNA gel for PCR reaction analysis of the expression of TGFβRII in BMDM postimmunoprecipitation for His. Right: data are representative of 3 independent experiments combined as fold over input (P = 0.006). B: THP-1 cells were transfected with empty vector (EV) or 1 μg of KLF10 plus TGF-βRII promoter reporter as described in materials and methods and analyzed by a dual-luciferase assay system. Cotransfection of a TGF-βRII promoter reporter that carries a mutation (Mut1) or a deletion (Del1) of the KLF10 binding site completely abrogates the promoter activity. The results represent the average of 3 transfection experiments performed in duplicate. Bottom: TGF-βRII promoter sequences with the respective deletion or mutation construct used for the transfection experiments.