Fig. 6.

Histone H3 acetylation is a KLF10-dependent event through recruitment of P300/CBP-associated factor (PCAF). A: ChiP assay of BMDM from KLF10^−/− or WT mice for acetylated histone H3 (H3A3) was performed as described in materials and methods. We observed lower histone H3 acetylation in the absence of KLF10 in BMDM. Right: data are representative of 4 independent experiments combined as fold over input (P = 0.001). B: PCAF is recruited to the TGFβRII promoter. BMDM were isolated from WT or KLF10^−/− mice and ChiP assays were performed by using anti-PCAF antibody. Primers flanking the core TGFβRII promoter were used to amplify the cross-linked DNA by semiquantitative PCR. Right: data are representative of 3 independent experiments combined as fold over input (P = 0.05).