Fig. 7.

Expression of CD44 and TLR4 in the small intestine. Immunofluorescence for A–D: CD44 (red) + Lgr5 (GFP, green). Immunofluorescence for E–H: TLR4 (red) + Lgr5 (GFP, green) in small intestinal crypt sections of postnatal day 14 mice. Some Lgr5⁺ crypt epithelial cells in WT mice also expressed CD44 (arrow) (A and B) and TLR4 (arrow) (E and F). Intestinal crypt sections of CD44^−/− and TLR4^−/− mice were used as negative controls for CD44 and TLR4 immunofluorescence, respectively. Scale bar for A, C, E, G = 50 μm, original magnification ×400. Scale bar for B, D, F, H = 20 μm, original magnification ×1,000.