Table 1.

Primers used for cloning and qPCR

	Primers
Name	Forward	Reverse (5′-3′)	Application	GenBank Accession Number
NaGLT1	5′-CTTTGTCTGGAGCCACCAAG-3′	5′-GCACGTGCTTTCACACCTTT-3′	Cloning	AB089802
SGLT1a	5′-AGCAGAGCTCCCAGATGTTG-3′	5′-ATAACAACTCCAGAGTCGCC-3′	Cloning	KT598255
UT-A2/UT-A2d	5′-TCGGGCATAGGAGACTTCAC-3′	5′-GAGGCTGCATTTACCAGACG-3′	Cloning	NM_001110270
UT-A2c	5′-GAGAGACAAACCAAAGAGCC-3′	5′-ATTTTGTTTCCATTCCACAGGC-3′	Cloning	KT598256
NaGLT1	5′-CTGCTCTTCCAAAGGTCCTG-3′	5′-TCGCTGTCTTCAGACACACC-3′	qPCR	AB089802
SGLT1a	5′-ACAAAATCGCCTGTGTCCTC-3′	5′-TCAGCACGAGGATGAACAAC-3′	qPCR	KT598255
UT-A1	5′-TCCTTCTCCTCACAAGCAAC-3′	5′-TCCTCCGTGTGAGTGTTCTC-3′	qPCR	NM_019347
UT-A2	5′-AGATGTCCAAGTGCCTTTGC-3′	5′-GGCCAGAGTGCTATTGAAGC-3′	qPCR	NM_001110270
UT-A2c	5′-TGACTCCATCTACTTTGGCC-3′	5′-CAGAAAGAGTTTTATTTTGTTTCCATT-3′	qPCR	KT598256
UT-A2d	5′-GCTCATCAAAGCCACGCTAG-3′	5′-GCTTCTGAGAGACAAACCAA-3′	qPCR	KT598257
ClC-K1	5′-TGGGGAAACTGAGAACAAGG-3′	5′-ATGGAGGTGATGGTCTCCTG-3′	qPCR	NM_053327
β-Actin	5′-AGCCATGTACGTAGCCATCC-3′	5′-TCTCAGCTGTGGTGGTGAAG-3′	qPCR	NM_031144
Cyclophilin A	5′-CGAGCTGTTTGCAGACAAAG-3′	5′-AGATGCCAGGACCTGTATGC-3′	qPCR	NM_017101

qPCR, quantitative real-time PCR; NaGLT1 and SGLT1a are Na⁺-dependent glucose transporters; UT, urea transporter; ClC-K1, Cl⁻ channel.