Fig. 1.

Pharmacological blockade of period (Per)1 nuclear entry in vivo results in decreased Na⁺/H+ exchanger (NHE)3 and Na⁺-glucose transporter (SGLT)1 expression but not SGLT2 expression in the renal cortex. Weight-matched male wild-type (WT) 129/sv mice were injected subcutaneously with vehicle (20% hydroxypropyl-β-cyclodextrin) or 30 mg/kg PF670462 [casein kinase (CK)1 isoforms δ/ε (CKinh)]every 12 h for 2.5 days starting at noon and euthanized at midnight 12 h after the last injection, as previously described (34). Kidneys were harvested, the cortex was dissected, and mRNA expresssion of NHE3 (A), SGLT1 (B), or SGLT2 (C) was measured by quantitative real-time PCR. Values are means ± SE; n = 4. *P < 0.05 compared with WT mice.