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Per1 knockdown results in decreased NHE3 and SGLT1 expression but not SGLT2 expression in HK-2 cells in vitro. HK-2 cells were treated with nontarget (NT) or Per1 small interfering (si)RNA for 48 h. Quantitative real-time PCR was used to evaluate changes in Per1 (A), NHE3 (B), SGLT1 (C), or SGLT2 (D) mRNA expression in Per1 siRNA versus NT siRNA controls. Values are means ± SE; n = 3. *P < 0.05; **P < 0.01.
