Fig. 4.

Pharmacological blockade of Per1 nuclear entry results in decreased nuclear Per1 in vitro. A: nuclear extracts were collected from HK-2 cells treated with 10 μM of the CK1δ/ε inhibitor PF670462 or water for 24 h. Western blot analysis was performed using anti-Per1 (nuclear Per1: ∼50 kDa) or anti-β-actin (∼42 kDa) antibodies as loading controls. Data are representative of three independent experiments. B: densitometry analysis was used to quantitate the level of Per1 in A. Values are means ± SE; n = 3. **P < 0.01.