TABLE 2 .
Activities of purified key metabolic enzymes
| Locus no. | Gene name | Enzyme activity | Activity (U/mg)a |
|---|---|---|---|
| CA_C3299 | bdhA | Butanol dehydrogenase | NADH (0.15 ± 0.05), NADPH (2.57 ± 0.45) |
| CA_C3298 | bdhB | Butanol dehydrogenase | NADH (0.18 ± 0.02), NADPH (2.95 ± 0.36) |
| CA_C3392 | bdhC | Butanol dehydrogenase | NADH (0.24 ± 0.04), NADPH (2.21 ± 0.41) |
| CA_P0162 | adhE1 | Butanol dehydrogenase | NADH (0.04 ± 0.02), NADPH (not detected) |
| CA_P0035 | adhE2 | Butanol dehydrogenase | NADH (4.8 ± 0.42), NADPH (0.12 ± 0.01) |
| CA_P0162 | adhE1 | Butyraldehyde dehydrogenase | NADH (2.27 ± 0.21), NADPH (0.08 ± 0.01) |
| CA_P0035 | adhE2 | Butyraldehyde dehydrogenase | NADH (2.5 ± 0.31), NADPH (0.07 ± 0.01) |
| CA_C2711-CA_C2709 | bcd-etfB-etfA | Butyryl-CoA dehydrogenase | NADH (0.569 ± 0.08), NADPH (not detected) |
| CA_C1673-CA_C1674 | gltA/gltB | Glutamate synthase | NADH (0.61 ± 0.16), NADPH (0.051 ± 0.01) |
| CA_C0737 | gdh | Glutamate dehydrogenase | NADH (41.2 ± 3.4), NADPH (0.12 ± 0.01) |
| CA_C0970 | citA | Re-citrate synthase | 1.9 ± 0.14 |
| CA_C0971 | citB | Aconitase | 6.5 ± 0.52 |
| CA_C0972 | citC | Isocitrate dehydrogenase | NADH (104 ± 6.8), NADPH (7.1 ± 0.43) |
| CA_C1589 | malS1 | Malic enzyme | NADH (156 ± 9.6), NADPH (3.4 ± 0.24) |
| CA_C1596 | malS2 | Malic enzyme | NADH (142 ± 12.7), NADPH (2.9 ± 0.34) |
a
One unit is the amount of enzyme that consumes 1 µmol of substrate per min.