TABLE 2 .
Current spectrum of popular, available NGS instruments (as of July 2015)a
| Instrument | Read length (accuracy) |
No. of reads/run |
Run time | Run cost ($) | No. of genomes/ metagenomes per run |
Comment |
|---|---|---|---|---|---|---|
| ILMN MiSeq | ≤300 bases (high up to 250 bases) |
≤20 million | ≤2.5 days | ≤1,500 | 40/4 | Read pairs |
| ILMN NextSeq | ≤150 bases (high) | ≤400 million | ≤1.2 days | ≤5,000 | 400/60 | Read pairs |
| ILMN HiSeq 2500 |
≤250 bases (high) | ≤300 million | 2.5 days | <8,000 | 300/80 | Read pairs, rapid run mode |
| PB RSII | ~10 kb (low for single pass) |
70,000 | ≤4 h | ≤1,200 | 3/TBD | Shorter (e.g., 2-kb) reads have higher quality; no paired ends |
| LT Ion PGM | ≤400 bases | ≤5 million | ≤7 h | ≤750 | 7/1 | PGM318 chip; no paired ends |
| LT Ion Proton | ≤200 bases | ≤80 million | ≤4 h | 1,000 | 30/16 | PI chip; no paired ends |
| ONT MinION | 10 kb–100 kb (low) | Variable | Variable | 1,000 | TBD | No paired ends; beta testing |
a
Abbreviations: ILMN, Illumina; LT, Life Technologies; PB, Pacific Biosciences; ONT, Oxford Nanopore Technologies. TBD, to be determined. ILMN is from Illumina. LT is from http://allseq.com/knowledgebank/sequencing-platforms/life-technologies-ion-torrent. Genomes/run, 100× coverage of a 3-Mb genome. Metagenomes/run, 5 million reads (pairs)/metagenome.