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. 2015 Dec 4;468(1-2):39–45. doi: 10.1016/j.bbrc.2015.10.169

Fig. 1.

Fig. 1

Wdr8 is a constitutive centrosomal protein enriched in the proximal end of the mother centriole. (A) HeLa cells were immunostained with antibodies against Wdr8 (green) and γ-tubulin (red). DAPI (4′,6-diamidino-2-phenylindole, blue) was used to visualise chromosomes. Enlarged images of the centrosomal region (squares on the top panels) are shown on the bottom. (B) Immunofluorescence images of HeLa cells stably expressing centrin-GFP using antibodies against Wdr8 (red), γ-tubulin (blue) and GFP (Centrin, green) during interphase (top) and mitosis (bottom) are shown. Only the centrosomal regions are dissected. Images of chromosomes (DAPI) are shown in the far-right panels. (C) Immunofluorescence images of HeLa cells stably expressing centrin-GFP using antibodies against Wdr8 (red), GFP (Centrin, green) and Cep135 (blue) are shown. Images of chromosomes (DAPI) are shown in the far-right panels. (D) Super resolution microscopy (OMX) images. HeLa cells stably expressing centrin-GFP were immunostained with antibodies against Wdr8 (left, red), GFP (Centrin, green) and γ-tubulin (blue) or Wdr8 (right, red), GFP (Centrin, green) and C-Nap (blue). Schematic presentation of the deduced localisation of individual proteins around the centrosomal region is shown on the right. Scale bars, 0.5 μm (D), 1 μm (A, bottom row and B and C), 5 μm (A, top row).