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. 2015 Dec 4;5:17498. doi: 10.1038/srep17498

Figure 6. Identification and differential expression of the genes encoding AMPs along the digestive tract of starved versus fed leeches.

Figure 6

(a) RT-PCR by using forward primer (TMC/NMC, TMZ or LUMB) with an oligodT was performed on RNA extracted from different dissected parts of the digestive tract one week after feeding and after one month of starvation. Amplified products were sub-cloned and sequenced. (b) NMC, LUMB and TMZ gene expressions were assessed by qRT-PCR in the intestine and the crop of starved leeches incubated with alive bacteria (A. veronii or A. hydrophila) or without (control) for 3 days. qRT-PCR results are presented as graphics. Graphics represent the best results of three independent experiments that displayed similar variations; p-values from Student’s T-tests were calculated between the different conditions, based on experimental measurements performed in triplicate (*p < 0.05).