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. 2015 Dec 4;5:17476. doi: 10.1038/srep17476

Figure 4. Gβ2γ5 and Gβ2γ11, but not Gβ2γ2 or PI3K are involved in histamine modulation of GR activity.

Figure 4

(A) HEK-293T cells co-transfected with TAT3-Luc, GR and H1R coding constructs were transfected with β2 and γ2; β2 and γ5; β2 and γ11 or not, and subjected to indicated treatments. Luciferase activity was determined as described in the methods section. Results are mean+/−SEM of at least three independent experiments performed in triplicates. **p < 0.01. (B) HEK-293T cells were co-transfected with TAT3-Luc, GR and H1R coding constructs and were treated with 2 μM LY294002 or 50 nM wortmannin 30 min prior to indicated treatments. Luciferase activity was determined as described in the methods section. Results are mean+/−SEM of at least three independent experiments performed in triplicates.