Figure 8. Antihistamines potentiate dexamethasone-induced GR activity and the overexpression of Gαtransducin does not inhibits their effects.

(A) HEK-293T cells co-transfected with the reporter TAT3-Luc, GR and H1R coding plasmids were preincubated for 10 min with increasing concentrations of mepyramine (Mep) or trans-triprolidine (Trip) and incubated with 0.1 nM dexamethasone for 24 h. (B) HEK-293T cells were co-transfected with TAT3-Luc, GR and H1R coding constructs and were transfected or not with Gαtransducin, as indicated, and subjected to treatments. Luciferase activity was determined as described in the methods section. Results are mean+/−SEM of three independent experiments performed in triplicates. *p < 0.05; **p < 0.01; ***p < 0.001.