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. 2015 Dec 4;5:17798. doi: 10.1038/srep17798

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Copyright © 2015, Macmillan Publishers Limited

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SP-D expression was analyzed by IHC (A–E) and Western blotting (F,G) methods. Pancreatic islets (A) and the intercalated duct system (B) stained positive for SP-D expression. As expected, there was no SP-D expression in the islet (C) and the intercalated duct system (D) of the pancreas in KO mice. Decreased SP-D expression was detected in the pancreatic islets and the intercalated duct system of septic mice (E). The results from Western blotting analysis show SP-D expression was observed in the pancreas of WT mice, but not SP-D KO mice (F). Furthermore, the levels of SP-D in the pancreas of septic WT mice 6 and 24 hrs after CLP and Sham controls were examined by Western blotting analysis. The results demonstrate decreased SP-D level in the pancreatic tissues of septic WT mice compared to Sham WT mice (p < 0.01) (G,H). Lung tissue was used as a positive control, (Magnification x400, scale bars = 50 μm).