Table 1.
Amplicons, primers and probes used for mutation scanning PIK3CA by DMA.
| Amplicon (exon, codons, size) | Primers (forward, reverse), probes (sense, antisense) | Sequences, sizes |
|---|---|---|
| PIK9 (exon 9, c. 542–546, 191 bp) | Primer PIK9-f | 5’-GGGGAAAAATATGACAAAG (19 b) |
| Primer PIK9-r | 5’-CATTTTAGCACTTACCTGTGAC (22 b) | |
| Probe PIK9-ROX(28)as | ROX-CTTTCTCCTGCTCAGTGATTTCAGAGAG-BHQ2 (28 b) | |
| PIK20 (exon 20, c. 1047–1049, 194 bp) | Primer PIK20-f | 5’-TGATGACATTGCATACATTC (20 bp) |
| Primer PIK20-r | 5’-TCCAGAGTGAGCTTTCATT (19 bp) | |
| Probe PIK20-Cy5(23)s | Cy5-ATGATGCACATCATGGTGGCTGG-BHQ2 (23 bp) |