Figure 2. Validation of age-associated methylation sites by using Sequenom MassARRAY in 50 healthy females.

(a) The reliability of the Sequenom MassARRAY output data. The confidence of methylation values for each product of primer per sample was assigned to a value referring to low (0) to high confidence (5). This value > 1.9 showed that the methylation level can be accepted. Our data from Sequenom MassARRAY is of high quality since 95% values were accepted. (b) Scatter plots of the methylation level as a function of age for 11 CpG sites that were selected from the Sequenom MassARRAY result at |R| < 0.5. The ID of CpG sites and their R values are shown in the right top corner of each sub-figure.