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. 2015 Jan 21;66(7):1877–1889. doi: 10.1093/jxb/eru530

Fig. 1.

Fig. 1.

Genomic organization of CFE1 genes in diploid and allotetraploid cotton. (A) Southern blot analysis of the gene isolated in this study from a normalized cDNA library in allotetraploid cotton. Genomic DNA was digested with HindIII, EcoRI, and XbaI, and hybridized with a 458bp fragment of the novel gene which contains one XbaI restriction site. (B) Gene structure analysis of CFE1 genomic sequences from G. herbaceum (A1), G. raimondii (D5), G. barbadense (AADD, Hai7124), and G. hirsutum (AADD, TM-1). The exons and introns are represented by different characters. (C) Phylogenetic analysis based on nucleic acid sequences of CFE1 genes from different cotton species. ‘GhCFE1A’, cDNA sequence isolated in this study; ‘GhCFE1D’, cDNA sequence isolated by Yamamoto and Baird (1998; originally named GhCFE1); ‘CFE1A A1’, genomic CFE1 sequence in G. herbaceum (A1); ‘CFE1D D5’, genomic CFE1 sequence in G. raimondii (D5); ‘CFE1A TM-1’ and ‘CFE1D TM-1’, A and D subgenome genomic CFE1 sequence in G. hirsutum TM-1(AADD), respectively; ‘CFE1A Hai7124’ and ‘CFE1D Hai7124’, A and D subgenome genomic CFE1 sequence in G. barbadense Hai7124 (AADD), respectively. (D) Chromosomal localization of GhCFE1D, the homeologous gene of GhCFE1A.