Fig. 3.

Mitotic metaphase spreads and karyotypes of RCC, 2 nF₂ and 4 nF₃ fish with FISH signals of 5S rDNA. (a) Two strong and a few weak signals were detected in RCC fish; the boxes indicated two major signals of 5S rDNA (green). (b) The karyotype of RCC fish revealed two strong signals located on the first pair of larger-sized SM chromosomes. (c) One strong signal and a few weak signals in 2nF₂ fish (green),the box indicated the one major signal of 5S rDNA (green). (d) The karyotype of 2nF₂ fish show one strong signal on the first group of larger-sized SM chromosomes. (e) The FISH analysis of 4nF₃ fish exhibited the double signal feature of two strong and a few weak signals (green), the boxes indicated the two major signals (green). (f ) The karyotype of 4nF₃ fish showed two strong signals on the first group of larger-sized SM chromosomes. All metaphase chromosomes were counterstained with DAPI and appear blue. (g) The chromosome pairing in Meiosis I of spermatocytes in 4nF₃ showed 100 bivalents under light microscope; (h) Two signals of 5S rDNA located on a separating bivalent. M: metacentric; SM: submetacentric; ST subtelelocentric; T: telocentric. (a-f) Bar = 3 μm; (g,h) Bar = 5 μm