Fig. 3.

Pharmacological inhibition of ALDH1A2-RAR signaling induces loss of cell-cell junctions and a mesenchymal-like phenotype. a Representative brigth field pictures of FaDu cells, which were treated with DMSO, 3 μM WIN18.446 or 3 μM BMS493 for the indicated time points. b FaDu cells were treated as described in (a) for the indicated time points and relative transcript levels of vimentin (VIM), fibronectin (FN1), N-cadherin (CDH2), E-cadherin (CDH1), Twist (TWIST1) and Snail (SNAI1) were assessed by quantitative RT-PCR using the ∆∆CT method. Transcript levels of ACTB were determined as a reference and bars represent mean value + SD of two independent experiments with three replicates. *p-value < 0.05, **p-value < 0.005, ***p-value < 0.0005