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Effect of RU-360 on the induction of apoptosis. The p53+/+ cells (A) and p53−/− cells (B) were treated with DHA or LA (50 μM) for 48 h and subsequently coincubated with 10 μM RU-360 (hatched bars) for 30 min. Following coincubation with fatty acid and butyrate for the final 12 h, the nucleosomal fragmentation assay was used to quantify apoptosis. Data are means ± SE, n = 6 wells per treatment. Bars not sharing a common letter are significantly different at P < 0.05.
