Table 1. List of the down-represented proteins in the cerebellum of Ugt1 mutant mice, as detected by 2-DE and identified by nanoLC-ESI-LIT-MS/MS analysis.
| Spot # | Protein identity | SwissProt entry | Peptides | Unique peptides | MASCOT score | EmPAI score | Sequence coverage (%) | Mw kDa | pI | MUT/WT ratio | Function |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Poly(rC)-binding protein (Pcbp1) | P60335 | 16 | 12 | 661 | 1.82 | 51.4 | 37.5 (38) | 6.66 (6.7) | 0.54 | Single-stranded nucleic acid binding protein that binds preferentially to oligo dC | |
| 3 | Alcohol dehydrogenase 5 (class III), chi polypeptide (Adh5) | Q6P5I3 | 6 | 6 | 336 | 0.31 | 17.9 | 39.6 (38) | 6.97 (6.7) | 0.54 | S-(hydroxymethyl)glutathione dehydrogenase activity and alcohol dehydrogenase (NAD) activity |
| Acetyl-CoA acetyltransferase (cytosolic) (Acat2) | Q8CAY6 | 4 | 4 | 184 | 0.29 | 18.1 | 41.3 (38) | 7.16 (6.7) | 0.54 | Catalysis of the reaction: 2 acetyl-CoA = CoA + acetoacetyl-CoA | |
| 4 | Isocitrate dehydrogenase [NAD] subunit α, mitochondrial (Idh3a) | Q9D6R2 | 14 | 10 | 400 | 0.71 | 27.9 | 36.7 (37) | 6.27 (5.5) | 0.56 | NAD binding, isocitrate dehydrogenase (NAD+) activity and magnesium ion binding |
| Guanine nucleotide-binding protein G(o) subunit α (Gnao) | P18872 | 6 | 6 | 325 | 0.70 | 18.4 | 40 (37) | 5.34 (5.5) | 0.56 | Modulators or transducers in various transmembrane signaling systems | |
| 5 | Peroxiredoxin-6 (Prdx6) | P30041 | 3 | 2 | 88 | 0.15 | 8.5 | 25 (25) | 6.00 (6.0) | 0.48 | Involved in redox regulation of the cell, may have a role in protection against oxidative injury |
| 6 | Superoxide dismutase [Cu-Zn] (Sod1) | P08228 | 8 | 8 | 379 | 1.95 | 44.8 | 15.8 (13) | 6.02 (5.9) | 0.48 | Destroys radicals that are normally produced within the cells and that are toxic to biological systems |
| 7 | Putative uncharacterized protein (Pfdn5) | Q6ZWY7 | 6 | 6 | 416 | 1.18 | 32.5 | 18 (10) | 5.95 (5.6) | 0.65 | Interacts selectively and non-covalently with an unfolded protein |
| 16 | Translationally controlled tumor protein (Tctp) | P63028 | 9 | 6 | 368 | 2.52 | 33.1 | 19.5 (20) | 4.76 (4.8) | 0.69 | Involved in calcium binding and microtubule stabilization |
| 17 | Peroxiredoxin-2 (Prdx2) | Q61171 | 9 | 6 | 434 | 2.61 | 31.8 | 21.8 (20) | 5.20 (5.1) | 0.65 | Involved in redox regulation of the cell, may have an important role in eliminating peroxides generated during metabolism |
| 18 | Parkinson disease (autosomal recessive, early onset) 7 (DJ-1) | B2KFH8 | 13 | 8 | 475 | 3.06 | 54.8 | 19.9 (20) | 6.32 (6.1) | 0.69 | Protects cells against oxidative stress and may act as an atypical peroxiredoxin-like peroxidase that scavenges H2O2. May function as a redox-sensitive chaperone |
| 19 | Phosphatidylethanolamine-binding protein 1 (Pebp1) | Q3TGC5 | 10 | 9 | 557 | 3.50 | 69.0 | 20.9 (19) | 5.36 (5.0) | 0.58 | Putative uncharacterized protein |
Spot number, protein description, accession number (SwissProt entry), theoretical and experimental (in parentheses) molecular mass and pI values, total and unique peptides detected, sequence coverage (%), MASCOT and EMPAI score values, fold increase with respect to control, and the known function of proteins are listed