Table 2. List of the over-represented proteins in the cerebellum of Ugt1 mutant mice, as detected by 2-DE and identified by nanoLC-ESI-LIT-MS/MS analysis.
| Spot # | Protein identity | SwissProt entry | Peptides | Unique peptides | MASCOT score | EmPAI score | Sequence coverage (%) | Mw kDa | pI | MUT/WT ratio | Function |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Dihydropyrimidinase-related protein 3 (Dpysl3) | Q3TT92 | 22 | 19 | 1081 | 1.39 | 48.2 | 62 (55) | 6.04 (6.0) | 1.72 | Catalysis of the hydrolysis of any non-peptide carbon-nitrogen bond in a cyclic amide | |
| 1 | Seryl-aminoacyl-tRNA synthetase (Sars) | Q8C483 | 13 | 11 | 522 | 0.42 | 25.6 | 61.2 (55) | 7.13 (6.0) | 1.72 | Catalysis of the reaction: ATP + L-serine + tRNA(Ser) = AMP + diphosphate + L-seryl-tRNA(Ser) |
| 2 | Putative uncharacterized protein (Vat1) | Q3TXD3 | 4 | 4 | 198 | 0.18 | 17.5 | 37.5 (47) | 6.66 (5.6) | 2.32 | Catalysis of a redox reaction; interacts selectively and non-covalently with zinc ions |
| 8 | V-type proton ATPase catalytic subunit A (Atp6v1a) | P50516 | 37 | 25 | 1536 | 3.15 | 45.7 | 68.3 (58) | 5.42 (5.3) | 1.72 | Catalytic subunit of the peripheral V1 complex of vacuolar ATPase; acidifies a variety of intracellular compartments in eukaryotic cells |
| 9 | Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial (Dlat) | Q8BMF4 | 20 | 14 | 788 | 0.7 | 25.7 | 68.0 (56) | 8.81 (5.6) | 2.00 | The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2 |
| Dihydropyrimidinase-related protein 3 (Dpysl3) | Q3TT92 | 7 | 6 | 380 | 0.42 | 16.0 | 61.8 (56) | 6.04 (5.6) | 2.00 | It is involved in nervous system development having hydrolase activity and acting on carbon–nitrogen bonds, in cyclic amides | |
| 10 | Dihydropyrimidinase-related protein 2 (Dpysl2) | O08553 | 18 | 13 | 866 | 1.24 | 31.1 | 62.3 (58) | 5.95 (5.8) | 1.56 | Necessary for signaling by class 3 semaphorins and cytoskeleton remodeling, axon guidance, neuronal growth and cell migration |
| 26S protease regulatory subunit 6B (Psmc4) | Q3TUN5 | 23 | 19 | 1089 | 1.89 | 54.1 | 47.4 (45) | 5.09 (5.1) | 1.66 | It has ATP binding and nucleoside-triphosphatase activity | |
| 11 | 26S protease regulatory subunit 6A (Psmc3) | Q3THI5 | 18 | 15 | 788 | 1.79 | 45.8 | 45.2 (45) | 5.39 (5.1) | 1.66 | It has ATP binding and nucleoside-triphosphatase activity |
| Enolase (Eno2) | Q3UJ20 | 13 | 11 | 669 | 0.83 | 32.5 | 47.3 (45) | 4.99 (5.1) | 1.66 | Belongs to the enolase family | |
| 12 | Transaldolase (Taldo) | Q93092 | 20 | 16 | 762 | 1.60 | 40.1 | 37.4 (33) | 6.57 (6.2) | 1.45 | It is important for the balance of metabolites in the pentose-phosphate pathway |
| LIM and SH3 domain protein 1 (Lasp1) | Q61792 | 9 | 8 | 419 | 1.09 | 34.2 | 29.9 (33) | 6.61 (6.2) | 1.45 | Has an important role in the regulation of dynamic actin-based, cytoskeletal activities | |
| 13 | 14-3-3 Protein epsilon (14-3-3e) | P62259 | 39 | 22 | 1405 | 32.90 | 72.2 | 29.2 (27) | 4.63 (4.5) | 1.82 | Adapter protein implicated in the regulation of both general and specialized signaling pathways |
| Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide (Ywhab) | A2A5N2 | 20 | 12 | 771 | 4.85 | 47.2 | 28.1 (27) | 4.77 (4.5) | 1.82 | It has monooxygenase activity and protein domain-specific binding | |
| 14 | 14-3-3 Protein epsilon (14-3-3e) | P62259 | 36 | 19 | 1268 | 29.02 | 66.7 | 29.2 (27) | 4.63 (4.6) | 1.58 | Adapter protein implicated in the regulation of both general and specialized signaling pathways |
| Tropomyosin alpha-4 chain (Tpm4) | Q6IRU2 | 14 | 13 | 741 | 1.71 | 52.4 | 28.5 (27) | 4.65 (4.6) | 1.58 | Binds to actin filaments in muscle and non-muscle cells, having a central role, with the troponin complex, in the calcium-dependent regulation of striated muscle contraction | |
| 15 | NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial (Nduv2) | Q9D6J6 | 12 | 10 | 647 | 1.80 | 48.8 | 27.3 (23) | 7.0 (5.3) | 1.40 | Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (complex I) |
| Apolipoprotein A-I (ApoA1) | Q3V2G1 | 11 | 8 | 457 | 1.26 | 28.8 | 30.7 (23) | 5.64 (5.3) | 1.40 | It has a role in lipid transport and binding |
Spot number, protein description, accession number (SwissProt entry), theoretical and experimental (in parentheses) molecular mass and pI values, total and unique peptides detected, sequence coverage (%), MASCOT and EMPAI score values, fold increase with respect to control, and the known function of proteins are listed