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. 2015 May 7;6(5):e1745. doi: 10.1038/cddis.2015.115

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Copyright © 2015 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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cPAF disrupts the cyclin-B1/CDK1 mitotic complex. (a) Cells were collected every 2-h post-cPAF treatment and expression of cyclin-B1, total CDK1 and phosphor-Cdc2(tyr15) determined by immunoblotting. (b) Nontreated control samples harvested at the same time points. (c) Expression of GADD45 in cPAF-treated and control cells. p84 expression was used as the loading control. (d) Blot density for cyclin-B1, total CDK, and phospho Cdc2(Tyr15), was determined using ImageJ on blots from control and cPAF-treated cells