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cPAF induces expression of pro-apoptotic markers. (a) HMC-1 cells were treated with 5 μg/ml of cPAF and the number of apoptotic cells was measured 24- and 48-h post-cPAF treatment. (b) HMC-1 cells were treated with 5 μg/ml of cPAF and samples were harvested at the indicated time points. Expression of cleaved Parp-1, cleaved Caspase and γ-H2AX was determined by Western analysis. p84 expression is used as the loading control
