Figure 1.

Che-1 binds to p53. (a) HCT116 cells were treated with 1μM Dox or vehicle for 8 h. Nuclear extracts were immunoprecipitated with anti-p53 or anti-Che-1 antibodies and analyzed by western blot (WB) using the indicated antibodies (abs). (b) MCF7 cells were treated as shown in (a) and nuclear extracts were immunoprecipitated with anti-p53 antibody and analyzed by WB with the indicated abs. (c) HCT116 cells were transiently transfected with expression vectors containing full-length p53 or its deletion mutants and treated with 1 μM Dox for 8 h. Total cell extracts (TCEs) were immunoprecipitated with anti-Che-1 antibody and analyzed by WB with the indicated abs. The bottom panel shows a schematic representation of a full-length p53 protein and its deletion mutants. (d) HCT116 cells were transiently transfected with expression vectors containing myc-tagged WT Che-1 or its deletion mutants and treated with 1 μM Dox for 8 h. TCEs were immunoprecipitated with anti-p53 antibody and analyzed by WB with the indicated abs. The bottom panel shows a schematic representation of a full-length Che-1 protein and its deletion mutants. (e) HCT116 cells were transiently transfected with expression vectors containing WT Che-1 or its deletion mutant ΔChe-1 and treated with 1 μM Dox for 8 h. TCEs were immunoprecipitated with anti-p53 or anti-Myc antibodies and analyzed by WB with the indicated abs