Figure 3.
RGD-modified oncolytic adenovirus exhibited enhanced infection ability and elicited cytotoxic effect on CAR-negative T24 sphere cells by inducing cell apoptosis. (a) Increased nucleic fragmentation (arrow) was observed in T24 sphere cells after 2-day treatment of OncoAd.RGD-hTERT-TRAIL (10 MOI) as detected by Hoechst staining, scale bar: 100 μm. (b) Statistic data for three repeats of (a). The percentage (%)=Number of nucleic fragmented cells in six fields/Number of total cells in six fields. (c) Two-day OncoAd.RGD-hTERT-TRAIL (10 MOI) treatment increased Sub-G1 population of T24 sphere cells. (d) Upregulation of PARP protein cleavage and downregulation of procaspase3 protein were observed in T24 sphere cells after treatment with OncoAd.RGD-hTERT-TRAIL (10 MOI) for 2 days. All the experiments were repeated three times and all data shown represented mean±S.D. (n=3). ***P<0.001