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. 2015 May 21;6(5):e1768. doi: 10.1038/cddis.2015.131

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Copyright © 2015 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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IAV NP and RNF43 co-localize in the nucleus of mammalian cells. (a) A549 cells were transfected with pEGFPN1 control plasmid or pEGFP-NP. Cells were fixed after 48 h and stained with DAPI for nucleus and anti-goat secondary antibody conjugated to Alexa-594 for RNF43 (red) and observed under confocal microscope. GFP-tagged NP is shown in green. (b) A549 cells were infected with PR8 IAV with 5 MOI and were fixed at the indicated time points. NP was stained using anti-NP monoclonal primary antibody and anti-mouse Alexa488 conjugated secondary antibody (green). RNF43 was stained using specific primary antibody and anti-rabbit Alexa-594 conjugated secondary antibody (red). Panels are labeled for their respective staining, RNF43 (red), NP (green) and nucleus (blue)