Figure 5.

EGCG opposes HBV-induced incomplete autophagy. (a) The effect of EGCG on p62 protein expression level. HepG2 cells were transfected with pUC19 or pHBV1.3. Forty-eight hours later, pHBV1.3-transfected cells were treated with or without 50 μM of EGCG for 24 h, and then subjected to western blotting using anti-p62 antibody. The expression of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as a loading control. (b) The effect of EGCG treatment on GFP-LC3 processing. GFP-LC3-transfected HepG2 cells were transfected with pUC19 or pHBV1.3; 48 h later, HBV-transfected cells were was treated with or without 50 μM of EGCG for 24 h, followed by the western blotting using anti-GFP. The expression of GAPDH was used as a loading control