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. 2015 May 7;6(5):e1755. doi: 10.1038/cddis.2015.95

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Copyright © 2015 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Markers of mitochondrial function and chromosomal alignment. (a) Mcl-1cKO (n=57) and Mcl-1^+/+ (n=85) MII oocytes were stained with a potential dependent mitochondrial dye (DePsipher). Values in graph (left) represent a proportion (%) of oocytes with few (<20) or numerous (>20) polarized (red) mitochondrial foci±S.E.M. per Mcl-1cKO or Mcl-1^+/+ oocytes. Values in graph (right) represent the proportion (%) of oocytes with mitochondrial distribution separated into pattern 1 or pattern 2±S.E.M. (b) Mean intensity of Mcl-1cKO (n=68) and Mcl-1^+/+ (n=52) MII oocytes stained with MitoTracker Red. Values represent average fold change±S.E.M. of relative fluorescence units (RFUs) per oocyte, normalized to wild type. (c) Mcl-1cKO (n=15) and Mcl-1^+/+ (n=15) oocytes were assayed for levels of TCA cycle substrates malate and fumarate. Values represent average metabolite level (μmol) per oocyte wet weight (kg)±S.E.M. (d) MII oocytes were stained with DCFDA (green) (Mcl-1cKO (n=20) and Mcl-1^+/+ (n=17)) or with MitoSox (red) (Mcl-1cKO (n=20) and Mcl-1^+/+ (n=24)). Values represent average fold change±S.E.M. of RFUs per oocyte, normalized to wild type. (e) MII oocytes were stained with DAPI (red) and anti-tubulin (green) to visualize chromatin and spindle; chromosomal misalignments (white arrow) from Mcl-1cKO (n=84) and Mcl-1^+/+ (n=89) oocytes were quantitated. Values represent percentage of oocytes with misaligned chromosomes/total oocyte pool. (*P<0.05, **P<0.01, ***P<0.001). Genotypes in legend apply to all graphs