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. 2015 Jan 8;6(1):e1588. doi: 10.1038/cddis.2014.551

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Copyright © 2015 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International Licence. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons licence, users will need to obtain permission from the licence holder to reproduce the material. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0

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Rif1 promoter shows Smad3-dependent H3K27 methylation ChIP-qPCR to examine (a) H3K9me2, (b) H3K9me3, (c) H3K4Me3, (d) H3K27Me3 and (e) Suz12 enrichment at Lefty1 and Lefty2 enhancer and Rif1 promoter in WT and Smad3−/− ESCs. Enriched Actin was analyzed as a negative control. The data are shown as the mean±S.D. (n=3). Statistically significant differences, calculated through student's t-tests, are indicated (*P<0.05; **P<0.01; ***P<0.001)