Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Jan 8;6(1):e1588. doi: 10.1038/cddis.2014.551

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2015 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International Licence. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons licence, users will need to obtain permission from the licence holder to reproduce the material. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0

PMC Copyright notice

Smad3−/− ESCs show enhanced cell proliferation and DNA repair capacity after UV irradiation. (a) Representative flow cytometry dot plot of BrdU-integrated WT (WT1 and WT2) and Smad3−/− (Smad3−/−1 and Smad3−/−2) ESCs at 0 and 6 h after UV irradiation. x axis represents DNA content through PI stain and y axis represents BrdU-FITC-labeled cells. (b) Statistical analysis of the BrdU-positive cell percentage of a from two independent experiments. The data are shown as the mean±S.D. (n=2). (c) Western blot and densitometric analyses of the expression of RPA2 in WT (WT1 and WT2) and Smad3−/− (Smad3−/−1 and Smad3−/−2) ESCs before, and at 1 and 3 h after, UV (40 mJ/cm²) irradiation. Gapdh expression level was used as an internal control. The densitometric data are shown as the mean±S.D. (n=2). (d) Western blot and densitometric analyses of the expression of pChk1 (Ser345) and pChk2 (Thr68) in WT (WT1 and WT2) and Smad3−/− (Smad3−/−1 and Smad3−/−2) ESCs before, and at 1 and 3 h after, UV (40 mJ/cm²) irradiation. Gapdh expression level was used as an internal control. The densitometric data are shown as the mean±S.D. (n=2). (e) Western blot and densitometric analyses of the expression of pH2AX (Ser139) in WT (WT1 and WT2) and Smad3−/− (Smad3−/−1 and Smad3−/−2) ESCs before, and at 6 h after, UV (40 mJ/cm²) irradiation. Gapdh expression level was used as an internal control. The data are shown as the mean±S.D. (n=2). Statistically significant differences, calculated through student's t-tests, are indicated (*P<0.05; **P<0.01; ***P<0.001)