| Actin F |
5′-ACCAACTGGGACGACATGGAGA-3′ |
| Actin R |
5′-TACGACCAGAGGCATACAGGGAC-3′ |
| Smad3 F |
5′-CTGGGCCTACTGTCCAATGT-3′ |
| Smad3 R |
5′-CATCTGGGTGAGGACCTTGT-3′ |
| Oct4 F |
5′-AAGCCTGCCAGGAGCAAA-3′ |
| Oct4 R |
5′-ATCCGGCGTTATGCTGCTCT-3′ |
| Nanog F |
5′-GGCTATCTGGTGAACGCATCTGGAAG-3′ |
| Nanog R |
5′-AACTGTACGTAAGGCTGCAGAAAGTCCTC-3′ |
| Rif1 F |
5′-ACTGTCTCCACGGATGAAGA-3′ |
| Rif1 R |
5′-CAAATAGCTGGCTTCCAGTG-3′ |
| Lefty1 F |
5′-TGTGTGTGCTCTTTGCTTCC-3′ |
| Lefty1 R |
5′-GGGGATTCTGTCCTTGGTTT-3′ |
| Lefty2 F |
5′-CAGCCAGAATTTTCGAGAGGT-3′ |
| Lefty2 R |
5′-CAGTGCGATTGGAGCCATC-3′ |
| Ccnd2 F |
5′-AAGCCTGCCAGGAGCAAA-3′ |
| Ccnd2 R |
5′-ATCCGGCGTTATGCTGCTCT-3′ |
| Cdx2 F |
5′-CCTGCGACAAGGGCTTGTTTAG-3′ |
| Cdx2 R |
5′-TCCCGACTTCCCTTCACCATAC-3′ |
| Pax6 F |
5′-GCATGCAGAACAGTCACAGCGGA-3′ |
| Pax6 R |
5′-ACTCCCGTTTATACTGGGCTATTT-3′ |
| Hand1 F |
5′-GCCAAGGATGCACAAGCA-3′ |
| Hand1 R |
5′-GGGCTGCTGAGGCAACTC-3′ |
| Fgf5 |
5′-GAGAGTGGTACGTGGCCCTGAACAAGAGAG-3′ |
| Fgf5 |
5′-CTTCAGTCTGTACTTCACTGGGCTGGGACT-3′ |
| T F |
5′-CATCGGAACAGCTCTCCAACCTAT-3′ |
| T R |
5′-GTGGGCTGGCGTTATGACTCA-3′ |
| Gata6 F |
5′-TGCAAGATTGCATCATGACAGA-3′ |
| Gata6 R |
5′-TGACCTCAGATCAGCCACGTTA-3′ |
| Sox17 F |
5′-TTCTGTACACTTTAATGAGGCTGTTC-3′ |
| Sox17 R |
5′-TTGTGGGAAGTGGGATCAAG-3′ |
| Cxcr4 F |
5′-AGCATGACGGACAAGTACC-3′ |
| Cxcr4 R |
5′-GATGATATGGA AGCCTTACAC-3′ |
| Mmp2 F |
5′-ATGATGACATCAAGGGGATC-3′ |
| Mmp2 R |
5′-CGCCAAATAAACCGGTCCTT-3′ |
| Mmp9 F |
5′-GAGCTGTGCGTCTTCCCCTTC-3′ |
| Mmp9 R |
5′-GGAATGATCTAAGCCCAGTGC-3′ |
| Eomes F |
5′-CCTGGTGGTGTTTTGTTGTG-3′ |
| Eomes R |
5′-TTTAATAGCACCGGGCACTC-3′ |
| Elf5 F |
5′-CCCTCCTCCTCTTCAAAACC-3′ |
| Elf5 R |
5′-AAGTTGCCACAAGACCATCC-3′ |
| Pdgfra F |
5′-ACGTTCAAGACCAGCGAGTT-3′ |
| Pdgfra R |
5′-CGATCGTTTCTCCTGC CTTA-3′ |
| CGA F |
5′-GCCAGAGTGGAGAATCATAC-3′ |
| CGA R |
5′-AACTGAAGCGCGTCAGAAGT-3′ |
| |
|
| ChIP-qPCR primers |
| Actin F |
5′-GTTACCCGGGATACTGACCT-3′ |
| Actin R |
5′-GGCACCACACCTTCTAC-3′ |
| Lefty1 F |
5′-GTAGCCAGCAGACAGGACAA-3′ |
| Lefty1 R |
5′-ATCCCCAATCCACATTCA-3′ |
| Lefty2 F |
5′-GCAATCTGCCCACTGTAAAA-3′ |
| Lefty2 R |
5′-TCGATCTTCCCAAGACTC-3′ |
| Rif1-1 F |
5′-CCAATTCTAGGCAGTTGCCT-3′ |
| Rif1-1 R |
5′-GGGAGTGTTGCTAAAGG-3′ |
| Rif1-2 F |
5′-ATCTCTGTGTTTGAGCACCC-3′ |
| Rif1-2 R |
5′-CGTGGAATCTTTCCGTCC-3′ |
| |
|
| shRNA sequences |
| Rif1 shRNA1 F |
5′-GATCCCCGAACCGTATTCAGAATCAAttcaagagaTTGATTCTGAATACGGTTCTTTTTA-3′ |
| Rif1 shRNA1 R |
5′-AGCTTAAAAAGAACCGTATTCAGAATCAAtctcttgaaTTGATTCTGAATACGGTTCGGG-3′ |
| Rif1 shRNA2 F |
5′-GATCCCCGAGTACAATAAGTGTTGATttcaagagaATCAACACTTATTGTACTCTTTTTA-3′ |
| Rif1 shRNA2 R |
5′-AGCTTAAAAAGAGTACAATAAGTGTTGATTctcttgaaaTCAACACTTATTGTACTCGGG-3′ |
| |
|
| Rif1 promoter primers |
| Rif1 F |
5′-GTGGTCACGCGTTGTAGTTCTGAGTCTCTGG-3′ |
| Rif1 R |
5′-ACGTCACTCGAGGCTAGAGATGGGTGATGTA-3′ |
| |
|
| cDNA clone primers |
| GFP F |
5′-ATACCGAGATCTATGGTGAGCAAGGGCGAGGAG-3′ |
| GFP R |
5′-ATACCCCTCGAGCTATCGAGATCTGAGTCCGGAC-3′ |
| Smad3 F |
5′-GTGGTCAGATCTATGTCGTCCATCCTGCCCT-3′ |
| Smad3 R |
5′-ACGTCACTCGAGCTAAGACACACTGGAACAGC-3′ |
| Pou5f1 F |
5′-GTGGTCACGCGTATGGCTGGACACCTGGCTT-3′ |
| Pou5f1 R |
5′-ACGTCACTCGAGTCAGTTTGAATGCATGGGAG-3′ |
| |
|
| Genotyping primers |
| pLvth-Rif1 shRNA F |
5′-CGCTGACGTCATCAACCCGCTCCAAGGA-3′ |
| pLvth-Rif1 shRNA R |
5′-CGTATAATGTATGCTATACGAAG-3′ |
