Figure 2.

Atg7 knockout did not affect oocytes number in fetal ovaries on 17.5 dpc. (a) Autophagy was severely disrupted in 17.5 dpc Atg7^F/F;Tnap-cre mice (n=3) ovaries by immunostaining of LC3. LC3 (in green), nuclear (in red). (b) Quantification of LC3 fluorescent intensity in panel (a). (c) Morphological analysis of 17.5 dpc fetal ovaries of Atg7^F/F;Tnap-cre mice and the control. Germ cell was indicated by immunohistochemical detection of MVH. (d) Quantification of oocytes number in 17.5 dpc fetal ovaries of Atg7^F/F;Tnap-cre mice (n=3) and the control (n=3). Data are presented as the mean±S.E.M. **P<0.01