Figure 5.

UBE3A catalytically inactive mutants inhibit HPV-16 E7 degradation of p130 and p107. HEK293 cells were transfected with HA-tagged HPV-16 E7, UBE3A, C833A, L502P, E550L (a and b), or p130 (a) or p107 (b), alone or in combination. After 24 h, cells were collected, and residual p130, p107 and UBE3A were detected by western blot analysis using either anti-HA antibody, or anti-UBE3A antibody. The expression of β-galactosidase (LacZ) was used as a control of transfection efficiency and loading