Figure 6.

UBE3A knockdown inhibits Mdm2 degradation of p53. p53 null H1299 cells were transfected with siRNA directed against luciferase (si luc) or UBE3A (si UBE3A). After 48 h, cells were transfected with p53 alone or in combination with Mdm2. After 72 h, cells were collected, and the levels of p53, UBE3A and the α-actinin and β-galactosidase (LacZ) loading controls were detected by western blotting