Figure 4.

MET is a direct target of miR-34c in NPC. (a) Wt or Mt miR-34c target sequences of the MET mRNA 3′-UTR. (b) Relative luciferase activity of CNE-2 and SUNE-1 cells after co-transfection with Wt or Mt MET 3′-UTR reporter genes (2 μg) and miR-34c mimic or miR-Ctrl (50 nM). Each experiment was independently repeated at least three times. Data are presented as mean±S.D.; **P<0.01 compared with the miR-Ctrl group, Student's t-test. (c and e) Quantification of MET mRNA expression by quantitative RT-PCR (c) and MET protein expression by western blotting (d) and immunofluorescent staining (e) after transfection with miR-34c mimic or miR-Ctrl. Each experiment was independently repeated at least three times. Data are presented as mean±S.D.; **P<0.01 compared with the miR-Ctrl group, Student's t-test