Figure 7.

Downregulation of miR-34c in NPC cells is associated with promoter hypermethylation. (a) Relative expression of miR-34c in NPC cell lines treated with (+) or without (−) DAC (2 μM). Each experiment was independently repeated at least three times. Data are presented as mean±S.D.; **P<0.01 compared with cells treated without (−) DAC, Student's t-test. (b) Average methylation level for seven CpG sites and (c) bisulfite pyrosequencing analysis of the miR-34c promoter region in CNE-2 and SUNE-1 cells treated with (+) or without (−) DAC. Data are presented as mean±S.D.; **P<0.01 compared with cells treated without (−) DAC, Student's t-test