Figure 6.

LMP2 knockdown by shRNA and MG132 pretreatment reduced infarction volumes in rats. The sections were stained by incubating them in a solution of 2% 2,3,5-triphenyltetrazolium chloride (TTC) and the infarcted brain tissue appeared white, whereas the non-infarcted region appeared red. LMP2 knockdown by shRNA significantly reduced infarction volumes in ischemic stroke in rats. Compared with MCAO+vehicle group (40.8%, infarction volumes/total ipsilateral hemispheric), the infarction volumes were reduced to 26.6% in MCAO+MG132 group (^▴P<0.001, compared with MCAO+vehicle group) (a and c). Similarly, the infarction volumes were reduced to 22.5% in LMP2-shRNA group compared with Cont-shRNA group (39.7%, infarction volumes/total ipsilateral hemispheric) (*P<0.001, compared with Cont-shRNA group) (b and d).The infarction volumes were smaller in LMP2-shRNA group than that in MG132 group although the difference did not reach a significant level (^#P>0.10 compared with MG132 group). Five rats in each group were used for TTC