Figure 6.

Protein levels in TAM67-negative versus -positive epidermis. (a) Suprabasal AP1 factor inactivation alters epidermal protein level. TAM67-rTA mice were treated for 7 days with 0 (C5, C7) or 2 (D3, D4) mg/ml doxycycline administered in drinking water. The epidermis was then separated from the dermis using dispase and equivalent protein amounts were electrophoresed on denaturing and reducing 10% polyacrylamide gel. The proteins were transferred to nitrocellulose for incubation with appropriate antibodies.⁷⁰ (b) Protein level as a function of time after AP1 factor inactivation. TAM67-rTA mice were treated for 0–7 days with 0 or 2 mg/ml doxycycline administered in drinking water. The level of the indicated proteins was then detected by immunoblot. Similar results were observed in each of three experiments