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. 2015 Feb 19;6(2):e1657. doi: 10.1038/cddis.2015.24

Figure 7.

Figure 7

HDAC8 overexpression counteracts ATRA treatment. (a) Longer-term colony assay (18 days) of BE(2)-C cells stably overexpressing HDAC8, treated with ATRA (10 μM), where indicated. Supernatant containing detached floating cells from densely grown untreated cells was transferred into a new 6-well plate and colonies were stained 10 days after replating. Results were quantified and are displayed in the bar diagram. Bars represent mean values and error bars represent S.E.M. (***P<0.001). SN, supernatant cells. (b) Hypothetical model: HDAC8 and ATRA signaling might converge at the level of CREB. Combined treatment of neuroblastoma cells with HDAC8 inhibitors and retinoic acid might enhance CREB-mediated differentiation. (c) Western blot analysis of BE(2)-C cells stably overexpressing HDAC8, where indicated, for P-CREB and CREB levels. (d) Bar diagram displays TH mRNA expression 72 h after treatment of BE(2)-C cells stably overexpressing HDAC8, where indicated, with ATRA (10 μM) (*P<0.05).