Figure 3.

Cortical soluble fractions from the PS1xAPP mice reduce FAIM-L levels and also induce death in primary cortical neurons. (a) Primary cortical neurons were treated for 48 h with different amounts of soluble fractions from WT or 6- or 18-month-old transgenic mice. FAIM-L and FAIM-S were determined by western blot, using pan-ERK and NeuN as a control of equal loading. (b) Quantification of three independent western blots (**P<0.01 and *P<0.05, t-test). (c) Neurons were treated for 48 h with the indicated amounts of the soluble fraction from WT and PS1xAPP mice of different ages, and the neuronal death induced was determined by counting nuclei after Hoechst staining. One-way ANOVA with multiple Newman–Keuls test gives ***P<0.001 between WT and 18 months with 100 μg/ml; and ^&&&P<0.001 and ^&&P<0.01 in relation to untreated neurons