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. 2015 Feb 12;6(2):e1639. doi: 10.1038/cddis.2015.6

Figure 5.

Figure 5

FAIM-L does not modulate Aβ toxicity. (a) Primary cortical neurons were treated with 10 μM of ADDL or the equivalent in vehicle in the presence or not of 40 μM of Q-VD for 48 h, and cell death was determined by counts of nuclear Hoechst staining. Data are mean±S.E.M. of three independent experiments. One-way ANOVA and Newman–Keuls post hoc. ***P<0.001 for comparison between untreated and vehicle-treated cells; ###P<0.001 ADDLs versus ADDLs+Q-VD. (b) Cortical neurons were transduced for 6 days with lentivirus (Lv) for downregulation (shFAIM-L) or overexpression (FAIM-L) of FAIM-L with the corresponding scrambled (sc) or empty controls. Cells were then treated with 5 μM ADDLs or the equivalent in vehicle for 48 h. Neuronal death was measured by counting nuclei stained with Hoechst. Data are mean±S.E.M. of three independent experiments. (c) FAIM-L immunoblot after infection. Loading control determined by Naphthol blue